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disc diffusion method  (Liofilchem)

 
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    Liofilchem disc diffusion method
    Disc Diffusion Method, supplied by Liofilchem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/disc+diffusion+method/diffusion+gradient+strips/pm41751514-55-45-48
    Average 86 stars, based on 1 article reviews
    disc diffusion method - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    AST Assay:

    Article Title: Stenotrophomonas maltophilia Infections in Haematological Malignancies and Hematopoietic Stem Cell Transplantation: A Case Series including Cefiderocol-Based Regimens
    Article Snippet: .. Cefiderocol AST was determined using disc diffusion method (Liofilchem, Italy) with a 30 μg disk incubated for 18–24 h at 35 ± 1 °C on Mueller–Hinton agar, as recommended by EUCAST. .. When necessary, zone diameter results were confirmed using lyophilised panels (Sensititre, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in Pseudomonas aeruginosa Isolated from Patients with Ventilator-Associated Pneumonia and Ventilator-Associated Tracheobronchitis in a Croatian Intensive Care Unit
    Article Snippet: Isolates were identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS, MALDI Biotyper sirius, Bruker, Bremen, Germany). .. Antimicrobial susceptibility testing (AST) was performed for antipseudomonal cephalosporins and penicillins (piperacillin/tazobactam (P/T), ceftazidime (CAZ), cefepime (CEF), ceftazidime/avibactam (CAZ/A), ceftolozane/tazobactam (C/T) and cefiderocol (FDC), quinolones (ciprofloxacin, CIP, and levofloxacin, LEV), carbapenems (imipenem (IMI) and meropenem, MEM) and aminoglycosides (amikacin (AMK) and tobramycin, TBR), using the disc diffusion method (Liofilchem, Roseto degli Abruzzi, Italy) and interpreted as susceptible (S), susceptible with increased exposure (I) or resistant (R), according to EUCAST (v. 13.0) [ ]. ..

    Article Title: Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in Pseudomonas aeruginosa Isolated from Patients with Ventilator-Associated Pneumonia and Ventilator-Associated Tracheobronchitis in a Croatian Intensive Care Unit.
    Article Snippet: Isolates were identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS, MALDI Biotyper sirius, Bruker, Bremen, Germany). .. Antimicrobial susceptibility testing (AST) was performed for antipseudomonal cephalosporins and penicillins (piperacillin/tazobactam (P/T), ceftazidime (CAZ), cefepime (CEF), ceftazidime/avibactam (CAZ/A), ceftolozane/tazobactam (C/T) and cefiderocol (FDC), quinolones (ciprofloxacin, CIP, and levofloxacin, LEV), carbapenems (imipenem (IMI) and meropenem, MEM) and aminoglycosides (amikacin (AMK) and tobramycin, TBR), using the disc diffusion method (Liofilchem, Roseto degli Abruzzi, Italy) and interpreted as susceptible (S), susceptible with increased exposure (I) or resistant (R), according to EUCAST (v. 13.0) [21]. ..

    Article Title: Stenotrophomonas maltophilia Infections in Haematological Malignancies and Hematopoietic Stem Cell Transplantation: A Case Series including Cefiderocol-Based Regimens.
    Article Snippet: .. Cefiderocol AST was determined using disc diffusion method (Liofilchem, Italy) with a 30 μg disk incubated for 18–24 h at 35 ± 1 ◦C on Mueller–Hinton agar, as recommended by EUCAST. .. When necessary, zone diameter results were confirmed using lyophilised panels (Sensititre, Thermo Fisher Scientific, Waltham, MA, USA).

    Diffusion-based Assay:

    Article Title: Stenotrophomonas maltophilia Infections in Haematological Malignancies and Hematopoietic Stem Cell Transplantation: A Case Series including Cefiderocol-Based Regimens
    Article Snippet: .. Cefiderocol AST was determined using disc diffusion method (Liofilchem, Italy) with a 30 μg disk incubated for 18–24 h at 35 ± 1 °C on Mueller–Hinton agar, as recommended by EUCAST. .. When necessary, zone diameter results were confirmed using lyophilised panels (Sensititre, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in Pseudomonas aeruginosa Isolated from Patients with Ventilator-Associated Pneumonia and Ventilator-Associated Tracheobronchitis in a Croatian Intensive Care Unit
    Article Snippet: Isolates were identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS, MALDI Biotyper sirius, Bruker, Bremen, Germany). .. Antimicrobial susceptibility testing (AST) was performed for antipseudomonal cephalosporins and penicillins (piperacillin/tazobactam (P/T), ceftazidime (CAZ), cefepime (CEF), ceftazidime/avibactam (CAZ/A), ceftolozane/tazobactam (C/T) and cefiderocol (FDC), quinolones (ciprofloxacin, CIP, and levofloxacin, LEV), carbapenems (imipenem (IMI) and meropenem, MEM) and aminoglycosides (amikacin (AMK) and tobramycin, TBR), using the disc diffusion method (Liofilchem, Roseto degli Abruzzi, Italy) and interpreted as susceptible (S), susceptible with increased exposure (I) or resistant (R), according to EUCAST (v. 13.0) [ ]. ..

    Article Title: Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in Pseudomonas aeruginosa Isolated from Patients with Ventilator-Associated Pneumonia and Ventilator-Associated Tracheobronchitis in a Croatian Intensive Care Unit.
    Article Snippet: Isolates were identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS, MALDI Biotyper sirius, Bruker, Bremen, Germany). .. Antimicrobial susceptibility testing (AST) was performed for antipseudomonal cephalosporins and penicillins (piperacillin/tazobactam (P/T), ceftazidime (CAZ), cefepime (CEF), ceftazidime/avibactam (CAZ/A), ceftolozane/tazobactam (C/T) and cefiderocol (FDC), quinolones (ciprofloxacin, CIP, and levofloxacin, LEV), carbapenems (imipenem (IMI) and meropenem, MEM) and aminoglycosides (amikacin (AMK) and tobramycin, TBR), using the disc diffusion method (Liofilchem, Roseto degli Abruzzi, Italy) and interpreted as susceptible (S), susceptible with increased exposure (I) or resistant (R), according to EUCAST (v. 13.0) [21]. ..

    Article Title: Stenotrophomonas maltophilia Infections in Haematological Malignancies and Hematopoietic Stem Cell Transplantation: A Case Series including Cefiderocol-Based Regimens.
    Article Snippet: .. Cefiderocol AST was determined using disc diffusion method (Liofilchem, Italy) with a 30 μg disk incubated for 18–24 h at 35 ± 1 ◦C on Mueller–Hinton agar, as recommended by EUCAST. .. When necessary, zone diameter results were confirmed using lyophilised panels (Sensititre, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Infectious spondylodiscitis: Epidemiology, diagnosis, microbiological findings, clinical features and outcomes in a 14-year retrospective study
    Article Snippet: .. For fastidious organisms and anaerobes, we used the disc diffusion method (Liofilchem ® ). .. Antimicrobial susceptibility testing was conducted with (Beckman Coulter Diagnostics).

    Article Title: Challenges Facing Two Outbreaks of Carbapenem-Resistant Acinetobacter baumannii : From Cefiderocol Susceptibility Testing to the Emergence of Cefiderocol-Resistant Mutants
    Article Snippet: .. The cefiderocol susceptibility tests were performed by the disc diffusion method (Liofilchem, Roseto degli Abruzzi, Italy) on Mueller–Hinton medium plates (MH agar plates), and new broth microdilution (BMD) commercially available tests, ComASP ® (Liofilchem, Roseto degli Abruzzi, Italy), in vitro according to the FDA drug approved label ( https://www.accessdata.fda.gov/cdrh_docs/reviews/K230479.pdf , accessed on 14 July 2024). ..

    Article Title: Infectious spondylodiscitis: Epidemiology, diagnosis, microbiological findings, clinical features and outcomes in a 14-year retrospective study.
    Article Snippet: .. For fastidious organisms and anaerobes, we used the disc diffusion method (Liofilchem®). .. Antimicrobial susceptibility testing was conducted with (Beckman Coulter Diagnostics).

    Incubation:

    Article Title: Stenotrophomonas maltophilia Infections in Haematological Malignancies and Hematopoietic Stem Cell Transplantation: A Case Series including Cefiderocol-Based Regimens
    Article Snippet: .. Cefiderocol AST was determined using disc diffusion method (Liofilchem, Italy) with a 30 μg disk incubated for 18–24 h at 35 ± 1 °C on Mueller–Hinton agar, as recommended by EUCAST. .. When necessary, zone diameter results were confirmed using lyophilised panels (Sensititre, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Stenotrophomonas maltophilia Infections in Haematological Malignancies and Hematopoietic Stem Cell Transplantation: A Case Series including Cefiderocol-Based Regimens.
    Article Snippet: .. Cefiderocol AST was determined using disc diffusion method (Liofilchem, Italy) with a 30 μg disk incubated for 18–24 h at 35 ± 1 ◦C on Mueller–Hinton agar, as recommended by EUCAST. .. When necessary, zone diameter results were confirmed using lyophilised panels (Sensititre, Thermo Fisher Scientific, Waltham, MA, USA).

    other:

    Article Title: "Evaluating the susceptibility of MBL carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. to ceftazidime/avibactam plus aztreonam and Cefiderocol: A synergy study and susceptibility profile".
    Article Snippet: This study assessed the in vitro susceptibility of aztreonam/avibactam (ATM/AVI) and the synergy analyses against 38 clinical carbapenemase-producing strains (31 Enterobacterales and 7 Pseudomonas spp.) collected over two years.. Susceptibility testing were performed followed EUCAST guidelines and synergy was studied using the gradient strip stacking method and FICI analysis.. Synergy between aztreonam (AZT) and Ceftazidime/Avibactam (CZA) was observed in 58,1 % of enterobacterales strains overall, with stronger synergy (100 %) in aztreonam-resistant group compared to aztreonamsusceptible ones (18.8 %).

    In Vitro:

    Article Title: Challenges Facing Two Outbreaks of Carbapenem-Resistant Acinetobacter baumannii : From Cefiderocol Susceptibility Testing to the Emergence of Cefiderocol-Resistant Mutants
    Article Snippet: .. The cefiderocol susceptibility tests were performed by the disc diffusion method (Liofilchem, Roseto degli Abruzzi, Italy) on Mueller–Hinton medium plates (MH agar plates), and new broth microdilution (BMD) commercially available tests, ComASP ® (Liofilchem, Roseto degli Abruzzi, Italy), in vitro according to the FDA drug approved label ( https://www.accessdata.fda.gov/cdrh_docs/reviews/K230479.pdf , accessed on 14 July 2024). ..



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